反应元件CRE和SRE参与猪CCR1和CCR5信号通路的分析

doi:10.11843/j.issn.0366-6964.2015.09.022

Abstract

Abstract:

The aim of the present study was to explore the signaling relationship between the chemokine regulated on activation，normal T cell expressed and secreted (RANTES) and its receptors［chemokine receptor 1(CCR1) and chemokine receptor 5(CCR5)］.The molecular cloning techniques and Western blotting were used to construct eukaryotic expression vectors，and then used dual luciferase report system tests to detect the expression of firefly luciferase after receptors activated or not with RANTES，to indirectly reflect the amount of cAMP response element(CRE) and serum response element(SRE) expression level.The results showed that CCR1 activated with RANTES could reduce the level of CRE expression and enhance the level of SRE expression in HEK293T cell，and CCR5 activated with RANTES could enhance the level of CRE expression and SRE expression.The results proved RANTES interacted with CCR1 could stimulate the level of SRE expression and reduce the level of CRE expression increased by Forskolin，with CCR5 could enhance the expression level of CRE and SRE.

CLC Number:

S852.23

CHAI Ji-tian，JIANG Jun-na，LI Dong，WANG Jian-bing，ZHOU Jia-hua，LI Ying-ying，WANG Zhi-qiang. Analysis of CRE and SRE Response Element in the Porcine CCR1 and CCR5 Signal Pathway[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1650-1655.

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Analysis of CRE and SRE Response Element in the Porcine CCR1 and CCR5 Signal Pathway

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